Embryonic Stem Cell Protocols Second Edition - Differentiation Models.pdf
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METHODS IN MOLECULAR BIOLOGY
™
330
Embryonic
Embryonic
Stem Cell
Stem Cell
Protocols
Protocols
Volume 2:
Differentiation Models
Volume 2:
Differentiation Models
S
ECOND
E
DITION
S
ECOND
E
DITION
Edited by
Kursad Turksen
Edited by
Kursad Turksen
Embryonic Stem Cell Protocols, Vol. 2
S
ECOND
E
DITION
M E T H O D S I N M O L E C U L A R B I O L O G Y
™
John M. Walker,
S
ERIES
E
DITOR
346.
Dictyostelium discoideum
Protocols
,
edited by
Ludwig Eichinger and Francisco Rivero-Crespo, 2006
345. Diagnostic Bacteriology Protocols
, Second
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edited by
Louise O'Connor, 2006
344. Agrobacterium Protocols
,
Second Edition:
Volume 2,
edited by
Kan Wang, 2006
343. Agrobacterium Protocols
,
Second Edition:
Volume 1,
edited by
Kan Wang, 2006
342. MicroRNA Protocols
,
edited by
Shao-Yao Ying, 2006
341. Cell–Cell Interactions:
Methods and Protocols,
edited by
Sean P. Colgan, 2006
340. Protein Design:
Methods and Applications,
edited by
Raphael Guerois and Manuela López de la
Paz, 2006
339. Microchip Capillary Electrophoresis:
Methods
and Protocols,
edited by
Charles Henry, 2006
338. Gene Mapping, Discovery, and Expression:
Methods and Protocols,
edited by
M. Bina, 2006
337. Ion Channels:
Methods and Protocols,
edited by
J. D. Stockand and Mark S. Shapiro, 2006
336. Clinical Applications of PCR:
Second Edition,
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Y. M. Dennis Lo, Rossa W. K. Chiu,
and K. C. Allen Chan, 2006
335. Fluorescent Energy Transfer Nucleic Acid
Probes:
Designs and Protocols,
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Vladimir
V. Didenko, 2006
334. PRINS and
In Situ
PCR Protocols:
Second
Edition,
edited by
Franck Pellestor, 2006
333. Transplantation Immunology:
Methods and
Protocols,
edited by
Philip Hornick and Marlene
Rose, 2006
332. Transmembrane Signaling Protocols:
Second
Edition,
edited by
Hydar Ali and Haribabu Bodduluri,
2006
331. Human Embryonic Stem Cell Protocols
,
edited
by
Kursad Turksen, 2006
330. Embryonic Stem Cell Protocols, Second Edition,
Vol. II: Differentiation Models,
edited by
Kursad
Turksen, 2006
329. Nonhuman Embryonic Stem Cell Protocols,
Second Edition,
Vol. I
:
Isolation and Character-
ization,
edited by
Kursad Turksen, 2006
328. New and Emerging Proteomic Techniques
,
edited by
Dobrin Nedelkov and Randall W. Nelson,
2006
327. Epidermal Growth Factor:
Methods and Protocols,
edited by
Tarun B. Patel and Paul J. Bertics, 2006
326.
In Situ
Hybridization Protocols,
Third Edition,
edited by
Ian A. Darby and Tim D. Hewitson,
2006
325. Nuclear Reprogramming:
Methods and Protocols,
edited by
Steve Pells, 2006
324. Hormone Assays in Biological Fluids,
edited by
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323. Arabidopsis Protocols,
Second Edition,
edited by
Julio Salinas and Jose J. Sanchez-Serrano, 2006
322.
Xenopus
Protocols:
Cell Biology and Signal
Transduction,
edited by
X. Johné Liu, 2006
321. Microfluidic Techniques:
Reviews and Protocols,
edited by
Shelley D. Minteer, 2006
320. Cytochrome P450 Protocols
, Second Edition,
edited
by
Ian R. Phillips and Elizabeth A. Shephard, 2006
319. Cell Imaging Techniques
, Methods and Protocols,
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Douglas J. Taatjes and Brooke T. Mossman,
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318. Plant Cell Culture Protocols
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edited
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Victor M. Loyola-Vargas and Felipe Vázquez-Flota,
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317. Differential Display Methods and Protocols
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Peng Liang, Jonathan Meade, and
Arthur B. Pardee, 2005
316. Bioinformatics and Drug Discovery
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315. Mast Cells:
Methods and Protocols,
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Guha
Krishnaswamy and David S. Chi, 2005
314. DNA Repair Protocols:
Mammalian Systems, Second
Edition,
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313. Yeast Protocols:
Second Edition,
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Wei
Xiao, 2005
312. Calcium Signaling Protocols:
Second Edition,
edited by
David G. Lambert, 2005
311. Pharmacogenomics:
Methods and Protocols,
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Federico Innocenti, 2005
310. Chemical Genomics:
Reviews and Protocols,
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Second Edition,
edited by
Anthony P. Davenport, 2005
305. Protein–Ligand Interactions:
Methods and
Applications,
edited by
G. Ulrich Nienhaus, 2005
304. Human Retrovirus Protocols:
Virology and
Molecular Biology,
edited by
Tuofu Zhu, 2005
303. NanoBiotechnology Protocols,
edited by
Sandra J.
Rosenthal and David W. Wright, 2005
302. Handbook of ELISPOT:
Methods and Protocols,
edited by
Alexander E. Kalyuzhny, 2005
301. Ubiquitin–Proteasome Protocols,
edited by
Cam Patterson and Douglas M. Cyr, 2005
300. Protein Nanotechnology:
Protocols,
Instrumentation, and Applications,
edited by
Tuan
Vo-Dinh, 2005
M E T H O D S I N M O L E C U L A R B I O L O G Y
™
Embryonic Stem Cell
Protocols
Volume 2: Differentiation Models
S
ECOND
E
DITION
Edited by
Kursad Turksen
Ottawa Health Research Institute
Ottawa, Ontario, Canada
© 2006 Humana Press Inc.
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Cover illustration: Figure 2D from Chapter 7 in Vol. 1, "Derivation and Propagation of Embryonic Stem
Cells in Serum- and Feeder-Free Culture," by Jennifer Nichols and Qi-Long Ying.
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Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1
eISBN: 1-59745-036-7
ISSN: 1064-3745
Library of Congress Cataloging-in-Publication Data
Embryonic stem cell protocols / edited by Kursad Turksen.-- 2nd ed.
v. ; cm. -- (Methods in molecular biology ; v. 329-330)
Includes bibliographical references and index.
Contents: v. 1. Isolation and characterization -- v. 2.
Differentiation models.
ISBN 1-58829-498-6 (v. 1 : alk. paper) -- ISBN 1-58829-784-5 (v. 2
: alk. paper)
1. Stem cells--Laboratory manuals. 2. Embryos--Laboratory manuals.
[DNLM: 1. Stem Cells. 2. Embryo Research. 3. Models, Animal.
QU 325 N812 2006] I. Turksen, Kursad. II. Series: Methods in
molecular biology (Clifton, N.J.) ; v. 329-330.
QH588.S83E46 2006
616'.02774--dc22
2005018023
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