Steroid Receptor Methods - Benjamin A. Lieberman.pdf - medyczne (angielski) - wojtek3101993

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M E T H O D S I N M O L E C U L A R B I O L O G Y TM

Steroid Receptor

Methods

Protocols and Assays

Edited by

Benjamin A. Lieberman

Blueprint Technologies, Englewood, CO

Humana Press

Totowa, New Jersey

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www.humanapress.com

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Library of Congress Cataloging in Publication Data

Steroid receptor methods : protocols and assays / edited by Benjamin A. Lieberman.

p. cm. -- (Methods in molecular biology ; 176)

Includes bibliographical references and index.

ISBN 0-89603-754-1 (alk. paper)

1. Steroid hormones--Receptors--Laboratory manuals. I. Lieberman, Benjamin A. II.

Series.

QP572.S7 .S756 2001

612.4'05--dc21

2001024304

Preface

This volume of the Methods in Molecular Biology series is entirely devoted

to the study of steroid receptor biology. Steroid hormone receptors represent a

powerful system for the study of both the most fundamental molecular mecha-

nisms of gene regulation and control and the gross physiological responses of

organisms to steroid hormones. Research in this field has brought forth advances

in the treatment of cancer, endocrine disorders, and reproductive biology, and

allowed elucidation of the fundamental biological mechanisms of gene expres-

sion. In Steroid Receptor Methods: Protocols and Assays , the reader will find a

collection of methods and protocols submitted by many fine steroid receptor

researchers from throughout the world. These authors have been instructed to

create a highly informative cross-section of the latest research techniques avail-

able. The resulting work is timely, useful, and approachable for both the expe-

rienced researcher and the novice to the field. Because the steroid receptor family

is represented by a wonderfully diverse, yet strongly interrelated set of steroid

receptor proteins, Steroid Receptor Methods contains protocols for the produc-

tion and purification of a variety of receptor forms, including the progesterone,

glucocorticoid, and androgen receptors. These procedures provide the raw mate-

rial needed to conduct sophisticated biochemical analysis of receptor properties.

Other techniques presented allow the reader to perform biochemical experiments

on DNA binding characteristics, hormone binding assays, and protocols using

combinatorial chemistry for drug discovery. Because steroid receptor effective-

ness is influenced by a variety of cellular proteins, there are included in this

volume a series of novel protocols utilizing the latest advances in immunochem-

istry, yeast two-hybrid screening, fluorescence, and other biochemical and

cellular techniques to detect and detail these interactions. These techniques

include both in vitro and in vivo approaches to provide the widest possible

selection of tools to the modern biological researcher. Finally, in recognition of

the growing importance of bioinformatics in biological research, several chapters

have been included to guide and assist the modern research biologist in harness-

ing this increasingly valuable resource. These chapters locate and make acces-

sible to the researcher the diverse computational tools currently available via the

Internet. Taken together these chapters provide both novice and experienced

researchers alike a set of invaluable tools to advance and extend their research.

Ben Lieberman, PhD

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