Laboratory Hematology Practice.pdf

Laboratory Hematology Practice

This book is dedicated to the memory of Dr Berend Houwen and Dr Noriyuki Tatsumi

Laboratory

Hematology Practice

EDITED BY

Kandice Kottke-Marchant MD, PhD

Chair, Pathology & Laboratory Medicine Institute

Professor and Chair, Department of Pathology, Cleveland Clinic Lerner College of Medicine

Section Head, Hemostasis and Thrombosis

Department of Clinical Pathology

Cleveland Clinic

Cleveland, OH, USA

WITH

Bruce H. Davis MD

Trillium Diagnostics, LLC,

Bangor, ME, USA

ISLH

International Society for

Laboratory Hematology

A John Wiley & Sons, Ltd., Publication

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Library of Congress Cataloging-in-Publication Data

Laboratory hematology practice / edited by Kandice Kottke-Marchant, Bruce H. Davis.

p. ; cm.

Includes bibliographical references and index.

ISBN-13: 978-1-4051-6218-0 (hard cover : alk. paper)

ISBN-10: 1-4051-6218-X (hard cover : alk. paper)

1. Hematology. 2. Blood–Analysis. I. Kottke-Marchant, Kandice. II. Davis, Bruce H., M.D.

[DNLM: 1. Hematologic Tests–methods. 2. Blood Chemical Analysis–methods. 3. Cytological

Techniques. 4. Hematologic Diseases–diagnosis. QY 400]

RB45.L235 2012

616.07'561–dc23

2011023990

A catalogue record for this book is available from the British Library.

Wiley also publishes its books in a variety of electronic formats. Some content that appears in print

may not be available in electronic books.

Set in 9 on 12 pt Meridien by Toppan Best-set Premedia Limited

1 2012

Contents

Contributors, viii

11 Validation and Quality Control in Clinical Flow

Cytometry, 115

Norman B. Purvis and Teri Oldaker

Preface, xiii

Abbreviations, xv

12 A New Paradigm for Cytometric Analysis, 131

C. Bruce Bagwell

I Cellular Analysis

III Molecular Diagnostics

1 Historical Perspective on Cellular Analysis, 3

Elkin Simson

13 Sample Collection, Processing, and Storage for

Molecular Genetic Testing, 145

Bobby L. Boyanton Jr. and Domnita Crisan

2 Cellular Morphologic Analysis of Peripheral Blood, 10

Powers Peterson, Sheila McNeill, and Gene Gulati

3 Automated Cell Analysis: Principles, 26

Bruce H. Davis and Patrick W. Barnes

14 Development of Ampliﬁ cation-Based Molecular

Genetic Testing in Hematology, 155

Raymond R. Tubbs

4 Differential Leukocyte Analysis, 33

Marie-Christine Béné and Francis Lacombe

15 Single Nucleotide Polymorphisms in Molecular

Diagnostics, 168

Gurunathan Murugesan, Seongsoo Jang, and Jin-Yeong Han

5 Automated Platelet Analysis, 48

Carol Briggs and Samuel J. Machin

6 Method Standardization in Cellular Analysis, 59

Ian Giles and Richard Kendall

16 Molecular Cytogenetic (FISH) Analysis of

Hematolymphoid Disorders, 181

Mark A. Micale and Anwar N. Mohamed

7 Spurious Counts and Spurious Results on Hematology

Analyzers: Platelets, 66

Marc Zandecki, Franck Genevieve, Jérémie Gérard, and

Alban Godon

17 Immunoglobulin and T-Cell Receptor Gene

Rearrangement Analysis for Diagnosis of Hematologic

Malignancies, 199

Jacques J. M. van Dongen, Anton W. Langerak, and

Tomasz Szczepa

8 Spurious Counts and Spurious Results on Hematology

Analyzers: White Blood Cells, Red Blood Cells,

Hemoglobin, Red Cell Indices, and Reticulocytes, 79

Marc Zandecki, Franck Genevieve, Jérémie Gérard, and

Alban Godon

ski

18 Minimal Residual Disease Detection in Acute Myeloid

Leukemia by RQ-PCR and Multiparameter Flow

Cytometry, 220

Wolfgang Kern and Susanne Schnittger

9 Evaluation of Hematology Analyzers, 96

Carol Briggs

19 Engraftment Analysis Using Short Tandem Repeats

Following Allogeneic Hematopoietic Cell

Transplantation, 234

Ilka Warshawsky and Hyun-Sook Chi

II Flow Cytometry in Hematologic Diagnosis

10 Flow Cytometric Specimen Collection, Processing, and

Reporting, 105

Maryalice Stetler-Stevenson, Bruce Greig, and Constance Yuan

20 Molecular Proﬁ ling Methods in the Diagnosis of

Hematologic Disorders, 244

Annette S. Kim, Stephen R. Master, and Cherie H. Dunphy

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